Characterization and virulence of Streptococcus agalactiae deficient in EIIA of the cellobiose-specific phosphotransferase system

Yundan Xie; Baotun Wang; Wei Li; Biao Jiang; Chun Liu; Jianmei Hu; Yanhua Huang; Bin Gong; Youlu Su

Aquaculture Reports (Jul 2021)

Characterization and virulence of Streptococcus agalactiae deficient in EIIA of the cellobiose-specific phosphotransferase system

Yundan Xie,
Baotun Wang,
Wei Li,
Biao Jiang,
Chun Liu,
Jianmei Hu,
Yanhua Huang,
Bin Gong,
Youlu Su

Affiliations

Yundan Xie: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China; College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China
Baotun Wang: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Wei Li: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China
Biao Jiang: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China
Chun Liu: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China
Jianmei Hu: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Yanhua Huang: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China
Bin Gong: The Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, Guangxi Province, China; Corresponding authors.
Youlu Su: Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong Province, China; Corresponding authors.

Journal volume & issue: Vol. 20
p. 100693

Abstract

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To indicate the EIIA protein of cellobiose-specific phosphotransferase transport system in affecting the characterization and virulence of Streptococcus agalactiae. A △cel-EIIA mutant was constructed from the wild-type S. agalactiae THN0901 strain by homologous recombination. In the present study, the cellobiose metabolism and biofilm formation of the △cel-EIIA mutant was significantly decreased, relative to that of the wild-type S. agalactiae THN0901. The proliferation capacity in vitro and the invasion and colonization ability in vivo of the △cel-EIIA mutant was also significantly lower than that of S. agalactiae THN0901. Furthermore, in tilapia infection model, the lethal dose 50 % the △cel-EIIA mutant was significantly higher than that of the wild strain. Taken together, the cel-EIIA gene plays a positive role in S. agalactiae cellobiose metabolism, biofilm formation and colonization of tilapia. The results of this study provide the basic data for the cellobiose metabolism and virulence mechanism in S. agalactiae of tilapia in the future.

Published in Aquaculture Reports

ISSN: 2352-5134 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Agriculture: Aquaculture. Fisheries. Angling
Website: http://www.journals.elsevier.com/aquaculture-reports/

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