Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in <i>Salix purpurea</i> and Expression Analysis in Response to Various Heavy Metal Stresses

Shuxin Chen; Juanjuan Chen; Zhuchou Lu; Yuhan Jia; Yuying Yang; Renying Zhuo; Xiaojiao Han

doi:10.3390/agronomy13092330

Agronomy (Sep 2023)

Genome-Wide Identification of Pleiotropic Drug Resistance (PDR) Transporters in <i>Salix purpurea</i> and Expression Analysis in Response to Various Heavy Metal Stresses

Shuxin Chen,
Juanjuan Chen,
Zhuchou Lu,
Yuhan Jia,
Yuying Yang,
Renying Zhuo,
Xiaojiao Han

Affiliations

Shuxin Chen: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Juanjuan Chen: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Zhuchou Lu: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Yuhan Jia: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Yuying Yang: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Renying Zhuo: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China
Xiaojiao Han: State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China

DOI: https://doi.org/10.3390/agronomy13092330
Journal volume & issue: Vol. 13, no. 9
p. 2330

Abstract

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Pleiotropic drug resistance (PDR) transporters, which are part of the ABCG subfamily of ATP-binding cassette (ABC) transporters, have been found to be involved in heavy metal tolerance. Salix species (willow) is widely regarded as a perfect candidate for phytoremediation of heavy metals because of its substantial biomass, strong tolerance, and remarkable capacity to accumulate heavy metals. However, the phylogeny and mechanisms underlying the response to heavy metals within the PDR family in willow have yet to be determined. In this study, we discovered and valuated a total of 21 PDR genes in the genome of Salix purpurea. The phylogenetic relationships of these genes were used to classify them into five major clades. The SpPDRs exhibited variations in exon-intron distribution patterns and gene lengths across different branches. Cis-acting elements linked to stress response, drought induction, low temperature, and defense response were discovered in the promoters of PDRs. Significant variations in the transcription levels of various PDR genes were observed across different tissues under heavy metal stress, with distinct heavy metals regulating different PDR members. In roots, PDR4 and PDR21 exhibited high expression levels. Meanwhile, PDR7 and PDR17 showed similar transcription patterns across all analyzed tissues. Furthermore, there was a significant and positive correlation between PDR5 and PDR16, whereas a significant and negative correlation was detected between PDR3 and PDR9, suggesting that the response of PDR members to heavy metals is complex and multifaceted. These findings will establish a vital basis for comprehending the biological functionalities of PDR genes, specifically their involvement in the regulation of willow’s tolerance to heavy metals.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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