Copper Oxide Nanoparticles Alter Serum Biochemical Indices, Induce Histopathological Alterations, and Modulate Transcription of Cytokines, <i>HSP70</i>, and Oxidative Stress Genes in <i>Oreochromis niloticus</i>
Hany M. R. Abdel-Latif,
Mahmoud A. O. Dawood,
Samy F. Mahmoud,
Mustafa Shukry,
Ahmed E. Noreldin,
Hanan A. Ghetas,
Mohamed A. Khallaf
Affiliations
Hany M. R. Abdel-Latif
Department of Poultry and Fish Diseases, Faculty of Veterinary Medicine, Alexandria University, Alexandria 21544, Egypt
Mahmoud A. O. Dawood
Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Samy F. Mahmoud
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Mustafa Shukry
Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
Ahmed E. Noreldin
Histology and Cytology Department, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, Egypt
Hanan A. Ghetas
Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt
Mohamed A. Khallaf
Department of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt
In the present study, fish were exposed to sub-lethal doses of CuONPs (68.92 ± 3.49 nm) (10 mg/L, 20 mg/L, and 50 mg/L) for a long exposure period (25 days). Compared to the control group (0.0 mg/L CuONPs), a significant dose-dependent elevation in blood urea and creatinine values, serum alanine transaminase, aspartate transaminase, and alkaline phosphatase enzyme activities were evident in CuONPs-exposed groups (p p < 0.05). Moreover, varying histopathological injuries were noticed in the hepatopancreatic tissues, posterior kidneys, and gills of fish groups correlated to the tested exposure dose of CuONPs. In summary, our results provide new insights and helpful information for better understanding the mechanisms of CuONPs toxicity in Nile tilapia at hematological, molecular levels, and tissue levels.
