iScience (Apr 2023)
Implantation of engineered human microvasculature to study human infectious diseases in mouse models
- Sophia Schönherr-Hellec,
- Eirini Chatzopoulou,
- Jean-Philippe Barnier,
- Yoann Atlas,
- Sébastien Dupichaud,
- Thomas Guilbert,
- Yves Dupraz,
- Julie Meyer,
- Catherine Chaussain,
- Caroline Gorin,
- Xavier Nassif,
- Stephane Germain,
- Laurent Muller,
- Mathieu Coureuil
Affiliations
- Sophia Schönherr-Hellec
- Université Paris Cité, UFR de Médecine, Paris, France; Institut Necker Enfants-Malades, Inserm U1151, CNRS UMR 8253, Paris, France
- Eirini Chatzopoulou
- Université Paris Cité, UPR2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant, UFR Odontologie, Paris, France
- Jean-Philippe Barnier
- Université Paris Cité, UFR de Médecine, Paris, France; Institut Necker Enfants-Malades, Inserm U1151, CNRS UMR 8253, Paris, France
- Yoann Atlas
- Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, PSL Research University, Paris, France; Sorbonne Université, Collège doctoral, Paris, France
- Sébastien Dupichaud
- Cell Imaging Platform, Structure Fédérative de Recherche Necker INSERM US24/CNRS UMS3633, Paris, France
- Thomas Guilbert
- Institut Cochin, INSERM U1016, CNRS UMR 8104, Université Paris Cité, Paris, France
- Yves Dupraz
- Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, PSL Research University, Paris, France
- Julie Meyer
- Université Paris Cité, UFR de Médecine, Paris, France; Institut Necker Enfants-Malades, Inserm U1151, CNRS UMR 8253, Paris, France
- Catherine Chaussain
- Université Paris Cité, UPR2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant, UFR Odontologie, Paris, France; AP-HP, Services Médecines bucco-dentaire (GH Paris Sud-Sorbonne Université, Paris Nord-Université Paris Cité), Paris, France
- Caroline Gorin
- Université Paris Cité, UPR2496 Pathologies, Imagerie et Biothérapies Orofaciales et Plateforme Imagerie du Vivant, UFR Odontologie, Paris, France; AP-HP, Services Médecines bucco-dentaire (GH Paris Sud-Sorbonne Université, Paris Nord-Université Paris Cité), Paris, France
- Xavier Nassif
- Université Paris Cité, UFR de Médecine, Paris, France; Institut Necker Enfants-Malades, Inserm U1151, CNRS UMR 8253, Paris, France
- Stephane Germain
- Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, PSL Research University, Paris, France
- Laurent Muller
- Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, PSL Research University, Paris, France; Corresponding author
- Mathieu Coureuil
- Université Paris Cité, UFR de Médecine, Paris, France; Institut Necker Enfants-Malades, Inserm U1151, CNRS UMR 8253, Paris, France; Corresponding author
- Journal volume & issue
-
Vol. 26,
no. 4
p. 106286
Abstract
Summary: Animal models for studying human pathogens are crucially lacking. We describe the implantation in mice of engineered human mature microvasculature consisting of endothelial and perivascular cells embedded in collagen hydrogel that allows investigation of pathogen interactions with the endothelium, including in vivo functional studies. Using Neisseria meningitidis as a paradigm of human-restricted infection, we demonstrated the strength and opportunities associated with the use of this approach.
