Nature Communications (Feb 2021)

Cas12a-assisted precise targeted cloning using in vivo Cre-lox recombination

  • Behnam Enghiad,
  • Chunshuai Huang,
  • Fang Guo,
  • Guangde Jiang,
  • Bin Wang,
  • S. Kasra Tabatabaei,
  • Teresa A. Martin,
  • Huimin Zhao

DOI
https://doi.org/10.1038/s41467-021-21275-4
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 11

Abstract

Read online

Direct cloning of biosynthetic gene clusters is difficult due to their size and repetitive nature. Here, the authors present CAPTURE, which uses Cas12a and in vivo Cre-lox recombination for efficient cloning of gene clusters up to 113 kb, and demonstrate how this method can be used for large-scale discovery of novel natural products.