Chinese Journal of Lung Cancer (Feb 2009)
Promotion of MAG-1 on Metastasis of Lung Cancer Cells in vitro and Its Expression in Lung Cancer Tissue of 24 Cases
Abstract
Background and objective Tumor metastasis is a multistep process with many genes involved in. A novel gene MAG-1 , identified by suppression subtractive hybridization from lung cancer cells was found to be associated with tumor metastasis. The aims of this work are to investigate the metastasis related effects of MAG-1 on human lunggiant-cell line PLA801, and to compare the expression rate of MAG-1 in cancer tissue from lung cancer patients with different metastatic status. Methods Sense and anti-sense expressing vectors of MAG-1 were constructed and transfected into PLA801C and PLA801D respectively. Colony forming, adherence assay, MTT assay and Transwell experiments were used to evaluate the alterations of clone forming, cell-matrix adherence, proliferating and invasion of the stable transfected cell strains. Western blot was employed to detect the proteins levels of CD44, and MMP-2 in cell strains and mRNAstate of MAG-1 in lung cancer tissue from patients with or without pathological metastasis were also analyzed by RTPCR. Results MAG-1 could increase cell-ECM (Extracellular Matrix) adhesive capacity, promote invasion, enhance cell proliferation and had no effects on clone forming ability of PLA801 cells in soft agar. MAG-1 was also found have positive effects on the protein level of CD44 and MMP-2 in PLA801 cells, and the detection rate of MAG-1 mRNA was much higher in cancer tissue from metastatic patients (7/12) than that in non-metastatic patients (2/12). Conclusion MAG-1 could promote lung cancer metastasis and might be a metastasis associated gene of lung cancer.
