The Effect of Hydroalcoholic Extract of Glaucium Flavum on the Activity of Anti Oxidative Enzymes in the Heart and Brain of Alloxan Induced Diabetic Rats

Abstract

BACKGROUND AND OBJECTIVE: Oxidative stress is the connection between diabetes and neuropathies and micro vascular disorders. This study was designed to investigate the effect of hydroalcoholic extract of Glaucium Flavum on the tissue activity of antioxidant enzymes in the heart and brain of Alloxan induced diabetic rats. METHODS: In this experimental study, 32 male rats were randomly divided into four groups of eight including control, diabetic, diabetic rats treated by Glaucium Flavum with dose of 500mg/kg and diabetic rats treated with Glibenclamide 5μg/kg. Diabetes was induced by single injection of 120mg/kg of Alloxan. After one month, activity of SOD, CAT and GPX were measured in the heart and brain tissues and analyzed. FINDINGS: Cardiac activity of all three enzymes in the diabetic + extract group were significantly higher than diabetic control (p<0.001). Activity of SOD in brain had a significant difference in comparison to diabetic + extract (8.79±1.4) and diabetic +drug groups (6.77±1.7) (p=0.03). As the same, CAT activity in diabetic+extract group (4.64±1.2) was significantly higher than diabetic + drug group (3.83±1.5) (p<0.001). Similar to the two previous state, GPX activity in diabetic+extract group (4.23±0.7) was significantly higher than diabetic+drug group (3.64±0.4) (p=0.03). CONCLUSION: The present study declared that yellow Glaucium Flavum extract can promote the main protective enzymatic mechanisms against diabetic induced oxidative stress in heart and brain. In addition, the effect of the extract was more successful than the effect of Glibenclamide and this effect was more pronounced in brain tissue.

Keywords

• diabetes mellitus
• glaucium flavum
• superoxide dismutase
• catalase
• glutathione peroxidase
• brain
• heart
• rat
• alloxan