Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

Haiyan Gao; Xinqi Gong; Jinchuan Zhou; Yubing Zhang; Jinsong Duan; Yue Wei; Liuqing Chen; Zixin Deng; Jiawei Wang; Shi Chen; Geng Wu; Lianrong Wang

doi:10.1038/s41467-022-34505-0

Nature Communications (Nov 2022)

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

Haiyan Gao,
Xinqi Gong,
Jinchuan Zhou,
Yubing Zhang,
Jinsong Duan,
Yue Wei,
Liuqing Chen,
Zixin Deng,
Jiawei Wang,
Shi Chen,
Geng Wu,
Lianrong Wang

Affiliations

Haiyan Gao: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Xinqi Gong: Mathematics Intelligence Application Lab, Institute for Mathematical Sciences, Renmin University of China
Jinchuan Zhou: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Yubing Zhang: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Jinsong Duan: State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University
Yue Wei: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Liuqing Chen: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, The Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University
Zixin Deng: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Jiawei Wang: State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University
Shi Chen: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University
Geng Wu: State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, The Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University
Lianrong Wang: Department of Gastroenterology, TaiKang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, School of Pharmaceutical Sciences, Wuhan University

DOI: https://doi.org/10.1038/s41467-022-34505-0
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 12

Abstract

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SspABCD–SspE is a phosphorothioation-sensing bacterial defence system. Here, authors find that SspE exploits DNA phosphorothioation-binding preference to modulate its biochemical activities for the self/nonself discrimination and phage resistance.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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