Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy

Sandra Muñoz-Braceras; Iago Pinal-Fernandez; Maria Casal-Dominguez; Katherine Pak; José César Milisenda; Shajia Lu; Massimo Gadina; Faiza Naz; Gustavo Gutierrez-Cruz; Stefania Dell’Orso; Jiram Torres-Ruiz; Josep Maria Grau-Junyent; Albert Selva-O’Callaghan; Julie J. Paik; Jemima Albayda; Lisa Christopher-Stine; Thomas E. Lloyd; Andrea M. Corse; Andrew L. Mammen

doi:10.3390/cells12172198

Cells (Sep 2023)

Identification of Unique microRNA Profiles in Different Types of Idiopathic Inflammatory Myopathy

Sandra Muñoz-Braceras,
Iago Pinal-Fernandez,
Maria Casal-Dominguez,
Katherine Pak,
José César Milisenda,
Shajia Lu,
Massimo Gadina,
Faiza Naz,
Gustavo Gutierrez-Cruz,
Stefania Dell’Orso,
Jiram Torres-Ruiz,
Josep Maria Grau-Junyent,
Albert Selva-O’Callaghan,
Julie J. Paik,
Jemima Albayda,
Lisa Christopher-Stine,
Thomas E. Lloyd,
Andrea M. Corse,
Andrew L. Mammen

Affiliations

Sandra Muñoz-Braceras: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Iago Pinal-Fernandez: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Maria Casal-Dominguez: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Katherine Pak: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
José César Milisenda: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Shajia Lu: Translational Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Massimo Gadina: Translational Immunology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Faiza Naz: Genomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Gustavo Gutierrez-Cruz: Genomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Stefania Dell’Orso: Genomic Technology Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Jiram Torres-Ruiz: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA
Josep Maria Grau-Junyent: Muscle Research Unit, Internal Medicine Service, Hospital Clinic de Barcelona, 08036 Barcelona, Spain
Albert Selva-O’Callaghan: Systemic Autoimmune Diseases Unit, Vall d’Hebron General Hospital, Universitat Autònoma de Barcelona, 08035 Barcelona, Spain
Julie J. Paik: Division of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Jemima Albayda: Division of Rheumatology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Lisa Christopher-Stine: Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Thomas E. Lloyd: Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Andrea M. Corse: Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
Andrew L. Mammen: Muscle Disease Unit, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA

DOI: https://doi.org/10.3390/cells12172198
Journal volume & issue: Vol. 12, no. 17
p. 2198

Abstract

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Dermatomyositis (DM), antisynthetase syndrome (AS), immune-mediated necrotizing myopathy (IMNM), and inclusion body myositis (IBM) are four major types of idiopathic inflammatory myopathy (IIM). Muscle biopsies from each type of IIM have unique transcriptomic profiles. MicroRNAs (miRNAs) target messenger RNAs (mRNAs), thereby regulating their expression and modulating transcriptomic profiles. In this study, 18 DM, 12 IMNM, 6 AS, 6 IBM, and 6 histologically normal muscle biopsies underwent miRNA profiling using the NanoString nCounter system. Eleven miRNAs were exclusively differentially expressed in DM compared to controls, seven miRNAs were only differentially expressed in AS, and nine miRNAs were specifically upregulated in IBM. No differentially expressed miRNAs were identified in IMNM. We also analyzed miRNA-mRNA associations to identify putative targets of differentially expressed miRNAs. In DM and AS, these were predominantly related to inflammation and cell cycle progression. Moreover, our analysis showed an association between miR-30a-3p, miR-30e-3p, and miR-199b-5p downregulation in DM and the upregulation of target genes induced by type I interferon. In conclusion, we show that muscle biopsies from DM, AS, and IBM patients have unique miRNA signatures and that these miRNAs might play a role in regulating the expression of genes known to be involved in IIM pathogenesis.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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