Tehran University Medical Journal (Oct 2016)

Evaluation of Brucella contamination in raw milk in Kerman dairy farms by PCR

  • Mohammad Khalili,
  • Mohammad Reza Aflatoonian,
  • Farzaneh Salari Aliabadi,
  • Jalil Abshenas

Journal volume & issue
Vol. 74, no. 7
pp. 0 – 0

Abstract

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Background: Human brucellosis is a significant public health problem in many middle east countries including Iran. In endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. The aim of this study was to detect brucellae in milk from dairy cattle farms in Kerman (Iran). Methods: Forthy and eight Bulk Tank Milk (BTM)  were collected from 48 dairy cattle farm including 4200 cow. All milk samples were examined by PCR to detect Brucella-specific DNA. Results: Using IS711 primer were detected in 4 samples (8.3%) Brucella spp from 48 BTM samples in this area.. Conclusions: The detection of Brucella DNA in milk for human consumption, especially the highly pathogenic species B. melitensis, is of obvious concern. The shedding of Brucella spp. in milk poses an increasing threat to consumers in Iran. Consumption of dairy products produced from non-pasteurized milk by individual farmers operating under poor hygienic conditions represents an unacceptable risk to public health.

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