Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex

Madison N. Wilson; Martin Thunemann; Xin Liu; Yichen Lu; Francesca Puppo; Jason W. Adams; Jeong-Hoon Kim; Mehrdad Ramezani; Donald P. Pizzo; Srdjan Djurovic; Ole A. Andreassen; Abed AlFatah Mansour; Fred H. Gage; Alysson R. Muotri; Anna Devor; Duygu Kuzum

doi:10.1038/s41467-022-35536-3

Nature Communications (Dec 2022)

Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex

Madison N. Wilson,
Martin Thunemann,
Xin Liu,
Yichen Lu,
Francesca Puppo,
Jason W. Adams,
Jeong-Hoon Kim,
Mehrdad Ramezani,
Donald P. Pizzo,
Srdjan Djurovic,
Ole A. Andreassen,
Abed AlFatah Mansour,
Fred H. Gage,
Alysson R. Muotri,
Anna Devor,
Duygu Kuzum

Affiliations

Madison N. Wilson: Department of Electrical and Computer Engineering, University of California San Diego
Martin Thunemann: Department of Biomedical Engineering, Boston University
Xin Liu: Department of Electrical and Computer Engineering, University of California San Diego
Yichen Lu: Department of Electrical and Computer Engineering, University of California San Diego
Francesca Puppo: Department of Pediatrics, University of California San Diego, School of Medicine
Jason W. Adams: Department of Pediatrics, University of California San Diego, School of Medicine
Jeong-Hoon Kim: Department of Electrical and Computer Engineering, University of California San Diego
Mehrdad Ramezani: Department of Electrical and Computer Engineering, University of California San Diego
Donald P. Pizzo: Department of Pathology, University of California San Diego
Srdjan Djurovic: Department of Medical Genetics, Oslo University Hospital
Ole A. Andreassen: NORMENT Center
Abed AlFatah Mansour: Laboratory of Genetics, The Salk Institute for Biological Studies
Fred H. Gage: Laboratory of Genetics, The Salk Institute for Biological Studies
Alysson R. Muotri: Department of Pediatrics, University of California San Diego, School of Medicine
Anna Devor: Department of Biomedical Engineering, Boston University
Duygu Kuzum: Department of Electrical and Computer Engineering, University of California San Diego

DOI: https://doi.org/10.1038/s41467-022-35536-3
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

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Neuronal organoids derived from human induced pluripotent stem cells can be transplanted and integrated into the rodent cortex for the study of brain development and function. Here the authors demonstrate use of transparent graphene microelectrodes and two photon imaging for longitudinal, multimodal monitoring of functional connectivity between human iPSC derived neuronal organoids and the mouse cortex.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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