Comprehensive characterization of evolutionary conserved breakpoints in four New World Monkey karyotypes compared to Chlorocebus aethiops and Homo sapiens
Xiaobo Fan,
Weerayuth Supiwong,
Anja Weise,
Kristin Mrasek,
Nadezda Kosyakova,
Alongkoad Tanomtong,
Krit Pinthong,
Vladimir A. Trifonov,
Marcelo de Bello Cioffi,
Pierre Grothmann,
Thomas Liehr,
Edivaldo H.C.de Oliveira
Affiliations
Xiaobo Fan
Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany
Weerayuth Supiwong
Department of Biology Faculty of Science, KhonKaen University, 123 Moo 16 Mittapap Rd., Muang District, KhonKaen 40002, Thailand
Anja Weise
Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany
Kristin Mrasek
Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany
Nadezda Kosyakova
Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany
Alongkoad Tanomtong
Department of Biology Faculty of Science, KhonKaen University, 123 Moo 16 Mittapap Rd., Muang District, KhonKaen 40002, Thailand
Krit Pinthong
Department of Biology Faculty of Science, KhonKaen University, 123 Moo 16 Mittapap Rd., Muang District, KhonKaen 40002, Thailand
Vladimir A. Trifonov
Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia
Marcelo de Bello Cioffi
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil
Pierre Grothmann
Serengeti-Park Hodenhagen GmbH, Am Safaripark 1, 29693, Hodenhagen, Germany
Thomas Liehr
Jena University Hospital, Friedrich Schiller University, Institute of Human Genetics, Kollegiengasse 10, D-07743 Jena, Germany
Edivaldo H.C.de Oliveira
Faculdade de Ciências Naturais, ICEN, Universidade Federal do Pará, Campus Universitário do Guamá, 66075-110 Belém-PA, Brazil
Comparative cytogenetic analysis in New World Monkeys (NWMs) using human multicolor banding (MCB) probe sets were not previously done. Here we report on an MCB based FISH-banding study complemented with selected locus-specific and heterochromatin specific probes in four NWMs and one Old World Monkey (OWM) species, i.e. in Alouatta caraya (ACA), Callithrix jacchus (CJA), Cebus apella (CAP), Saimiri sciureus (SSC), and Chlorocebus aethiops (CAE), respectively. 107 individual evolutionary conserved breakpoints (ECBs) among those species were identified and compared with those of other species in previous reports. Especially for chromosomal regions being syntenic to human chromosomes 6, 8, 9, 10, 11, 12 and 16 previously cryptic rearrangements could be observed. 50.4% (54/107) NWM-ECBs were colocalized with those of OWMs, 62.6% (62/99) NWM-ECBs were related with those of Hylobates lar (HLA) and 66.3% (71/107) NWM-ECBs corresponded with those known from other mammalians. Furthermore, human fragile sites were aligned with the ECBs found in the five studied species and interestingly 66.3% ECBs colocalized with those fragile sites (FS). Overall, this study presents detailed chromosomal maps of one OWM and four NWM species. This data will be helpful to further investigation on chromosome evolution in NWM and hominoids in general and is prerequisite for correct interpretation of future sequencing based genomic studies in those species.
