Nature Communications (Jul 2020)

Fast three-color single-molecule FRET using statistical inference

  • Janghyun Yoo,
  • Jae-Yeol Kim,
  • John M. Louis,
  • Irina V. Gopich,
  • Hoi Sung Chung

DOI
https://doi.org/10.1038/s41467-020-17149-w
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 14

Abstract

Read online

Three-colour FRET is a powerful tool to study macromolecular conformational dynamics, but is temporally limited due to the experimental complexity. Here the authors develop experimental and analytical methods for probing submillisecond-time scale dynamics using single continuous-wave excitation.