Foods (Oct 2022)

Influence of Emulsifying Salts on the Growth of <i>Bacillus thuringiensis</i> CFBP 3476 and <i>Clostridium perfringens</i> ATCC 13124 in Processed Cheese

  • Andressa Fusieger,
  • Raiane Rodrigues da Silva,
  • Valéria Quintana Cavicchioli,
  • Rafaela da Silva Rodrigues,
  • Jaqueline Aparecida Honorato,
  • Sidney Rodrigues de Jesus Silva,
  • Mariana Lage Pena,
  • Cinzia Caggia,
  • Luís Augusto Nero,
  • Antonio Fernandes de Carvalho

DOI
https://doi.org/10.3390/foods11203217
Journal volume & issue
Vol. 11, no. 20
p. 3217

Abstract

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Processed cheese is a dairy product with multiple end-use applications, where emulsifying salts play a fundamental role in physicochemical changes during production. Moreover, some of these salts may be a strategy to control spoilage and pathogenic microorganisms, contributing to safety and shelf life extension. This study aimed to evaluate the in vitro inhibitory activity of two emulsifying salts (ESSP = short polyP and BSLP = long polyP) against Bacillus thuringiensis CFBP 3476 and Clostridium perfringens ATCC 13124, and to compare the in situ effects of two emulsifying salts treatments (T1 = 1.5% ESSP and T2 = 1.0% ESSP + 0.5% BSLP) in processed cheeses obtained by two different methods (laboratory- and pilot-scales), during 45-day storage at 6 °C. C. perfringens ATCC 13124 growth was not affected in vitro or in situ (p > 0.05), but both of the treatments reduced B. thuringiensis CFBP 4376 counts in the tested condition. Counts of the treatments with B. thuringiensis CFBP 3476 presented a higher and faster reduction in cheeses produced by the laboratory-scale method (1.6 log cfu/g) when compared to the pilot-scale method (1.8 log cfu/g) (p B. thuringiensis CFBP 4376 growth reduction.

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