Restoration of plasma kidney and liver biomarkers in doxorubicin-treated Wistar rats by aqueous extracts of Pleurotus tuberregium sclerotia and Cnidoscolus aconitifolius leaves

Abstract

The ability of aqueous extracts of sclerotia of Pleurotus tuberregium and leaves of Cnidoscolus aconitifolius to regulate plasma markers of kidney and liver function/integrity was investigated in doxorubicin-treated Wistar rats. Doxorubicin (dissolved in normal saline) was injected intraperitoneally (15 mg/kg body weight) into the rats; metformin was daily administered orally at 250 mg/kg, while the extracts were daily administered orally at doses of 50, 75, and 100 mg/kg. Compared to the test control, in both the doxorubicin pre-treatment (or ameliorative) study and the extract pre-treatment (protective) studies, the extracts and metformin-treated groups had significantly lower (P < 0.05) plasma levels of alkaline phosphatase, alanine transaminase and aspartate transaminase, and concentrations of creatinine, urea, and blood urea nitrogen. However, the plasma globulin, albumin, and total protein concentrations and the albumin/globulin ratio of the extract and metformin-treated groups were significantly higher (P < 0.05). The extracts prevented (in the protective study) or attenuated (in the ameliorative study) doxorubicin-induced increase in the levels of plasma markers of kidney and liver function/integrity, and afforded protection or recovery towards near-normal values.

Keywords

• albumin/globulin ratio
• alkaline phosphatase
• blood urea nitrogen
• creatinine
• doxorubicin
• transaminases