Antioxidants (Feb 2021)

Tyrosine Phosphorylation Modulates Peroxiredoxin-2 Activity in Normal and Diseased Red Cells

  • Alessandro Mattè,
  • Enrica Federti,
  • Elena Tibaldi,
  • Maria Luisa Di Paolo,
  • Giovanni Bisello,
  • Mariarita Bertoldi,
  • Andrea Carpentieri,
  • Pietro Pucci,
  • Iana Iatcencko,
  • Anand B. Wilson,
  • Veronica Riccardi,
  • Angela Siciliano,
  • Francesco Turrini,
  • Dae Won Kim,
  • Soo Young Choi,
  • Anna Maria Brunati,
  • Lucia De Franceschi

DOI
https://doi.org/10.3390/antiox10020206
Journal volume & issue
Vol. 10, no. 2
p. 206

Abstract

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Peroxiredoxin-2 (Prx2) is the third most abundant cytoplasmic protein in red blood cells. Prx2 belongs to a well-known family of antioxidants, the peroxiredoxins (Prxs), that are widely expressed in mammalian cells. Prx2 is a typical, homodimeric, 2-Cys Prx that uses two cysteine residues to accomplish the task of detoxifying a vast range of organic peroxides, H2O2, and peroxynitrite. Although progress has been made on functional characterization of Prx2, much still remains to be investigated on Prx2 post-translational changes. Here, we first show that Prx2 is Tyrosine (Tyr) phosphorylated by Syk in red cells exposed to oxidation induced by diamide. We identified Tyr-193 in both recombinant Prx2 and native Prx2 from red cells as a specific target of Syk. Bioinformatic analysis suggests that phosphorylation of Tyr-193 allows Prx2 conformational change that is more favorable for its peroxidase activity. Indeed, Syk-induced Tyr phosphorylation of Prx2 enhances in vitro Prx2 activity, but also contributes to Prx2 translocation to the membrane of red cells exposed to diamide. The biologic importance of Tyr-193 phospho-Prx2 is further supported by data on red cells from a mouse model of humanized sickle cell disease (SCD). SCD is globally distributed, hereditary red cell disorder, characterized by severe red cell oxidation due to the pathologic sickle hemoglobin. SCD red cells show Tyr-phosphorylated Prx2 bound to the membrane and increased Prx2 activity when compared to healthy erythrocytes. Collectively, our data highlight the novel link between redox related signaling and Prx2 function in normal and diseased red cells.

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