Nature Communications (May 2018)

Phenotypic diversification by enhanced genome restructuring after induction of multiple DNA double-strand breaks

  • Nobuhiko Muramoto,
  • Arisa Oda,
  • Hidenori Tanaka,
  • Takahiro Nakamura,
  • Kazuto Kugou,
  • Kazuki Suda,
  • Aki Kobayashi,
  • Shiori Yoneda,
  • Akinori Ikeuchi,
  • Hiroki Sugimoto,
  • Satoshi Kondo,
  • Chikara Ohto,
  • Takehiko Shibata,
  • Norihiro Mitsukawa,
  • Kunihiro Ohta

DOI
https://doi.org/10.1038/s41467-018-04256-y
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 15

Abstract

Read online

DNA double-strand break (DSB) leads to genome rearrangements with various genetic and phenotypic effects. Here, the authors develop a tool to induce large-scale genome restructuring by introducing conditional multiple DNA breaks, and produce various traits in yeast and Arabidopsis thaliana.