IUCrJ (Mar 2014)
Femtosecond X-ray diffraction from two-dimensional protein crystals
- Matthias Frank,
- David B. Carlson,
- Mark S. Hunter,
- Garth J. Williams,
- Marc Messerschmidt,
- Nadia A. Zatsepin,
- Anton Barty,
- W. Henry Benner,
- Kaiqin Chu,
- Alexander T. Graf,
- Stefan P. Hau-Riege,
- Richard A. Kirian,
- Celestino Padeste,
- Tommaso Pardini,
- Bill Pedrini,
- Brent Segelke,
- M. Marvin Seibert,
- John C. H. Spence,
- Ching-Ju Tsai,
- Stephen M. Lane,
- Xiao-Dan Li,
- Gebhard Schertler,
- Sebastien Boutet,
- Matthew Coleman,
- James E. Evans
Affiliations
- Matthias Frank
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- David B. Carlson
- Department of Molecular and Cellular Biology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
- Mark S. Hunter
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- Garth J. Williams
- Linac Coherent Light Source, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Marc Messerschmidt
- Linac Coherent Light Source, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Nadia A. Zatsepin
- Arizona State University, 300 East University Drive, Tempe, AZ 85287, USA
- Anton Barty
- Center for Free-Electron Laser Science, University of Hamburg, Luruper Chaussee 149, Hamburg 22761, Germany
- W. Henry Benner
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- Kaiqin Chu
- Center for Biophotonics, 2700 Stockton Boulevard, Sacramento, CA 95817, USA
- Alexander T. Graf
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- Stefan P. Hau-Riege
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- Richard A. Kirian
- Center for Free-Electron Laser Science, University of Hamburg, Luruper Chaussee 149, Hamburg 22761, Germany
- Celestino Padeste
- Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
- Tommaso Pardini
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- Bill Pedrini
- Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
- Brent Segelke
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- M. Marvin Seibert
- Linac Coherent Light Source, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- John C. H. Spence
- Arizona State University, 300 East University Drive, Tempe, AZ 85287, USA
- Ching-Ju Tsai
- Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
- Stephen M. Lane
- Center for Biophotonics, 2700 Stockton Boulevard, Sacramento, CA 95817, USA
- Xiao-Dan Li
- Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
- Gebhard Schertler
- Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
- Sebastien Boutet
- Linac Coherent Light Source, 2575 Sand Hill Road, Menlo Park, CA 94025, USA
- Matthew Coleman
- Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550, USA
- James E. Evans
- Department of Molecular and Cellular Biology, University of California, Davis, 1 Shields Avenue, Davis, CA 95616, USA
- DOI
- https://doi.org/10.1107/S2052252514001444
- Journal volume & issue
-
Vol. 1,
no. 2
pp. 95 – 100
Abstract
X-ray diffraction patterns from two-dimensional (2-D) protein crystals obtained using femtosecond X-ray pulses from an X-ray free-electron laser (XFEL) are presented. To date, it has not been possible to acquire transmission X-ray diffraction patterns from individual 2-D protein crystals due to radiation damage. However, the intense and ultrafast pulses generated by an XFEL permit a new method of collecting diffraction data before the sample is destroyed. Utilizing a diffract-before-destroy approach at the Linac Coherent Light Source, Bragg diffraction was acquired to better than 8.5 Å resolution for two different 2-D protein crystal samples each less than 10 nm thick and maintained at room temperature. These proof-of-principle results show promise for structural analysis of both soluble and membrane proteins arranged as 2-D crystals without requiring cryogenic conditions or the formation of three-dimensional crystals.
Keywords
- two-dimensional protein crystal
- femtosecond crystallography
- single layer X-ray diffraction
- membrane protein
