Change the original microbial community structure in the hydrolysis acidification tank to enhance the COD removal performance of oily wastewater

Abstract

The hydrolysis acidification tank mainly relies on microorganisms to treat oily sewage, but in many cases the chemical oxygen demand (COD) of the effluent from the hydrolysis acidification tank does not decrease or even increase. In this work, about 50 L of oily wastewater is treated in a facultative anaerobic hydrolysis acidification tank with a temperature of 29 °C, pH 6, high-throughput sequencing technology analyzes found that after long-term operation of the hydrolysis and acidification tank, the dominant bacterial Pseudomonas accounted for only 2.87%, at this time, the effluent COD of the hydrolysis and acidification tank was 450 mg/L. Pseudomonas stutzeri LH-42 a strain screened in the laboratory, was domesticated and colonized in the hydrolysis acidification tank. High-throughput sequencing and bioinformatics analysis showed that the proportion of Pseudomonas in the hydrolysis acidification tank reached 5.89%, the effluent COD of the hydrolysis and acidification tank was 200 mg/L. The above results indicate the importance of the proportion of Pseudomonas in the hydrolysis and acidification tank for the COD degradation of oily wastewater. HIGHLIGHTS After the bacteria intensification, the COD removal rate of the oily wastewater by the hydrolysis acidification reactor increased by 50%.; The biodegradability of wastewater has also been correspondingly improved.; The microbial community structure in the hydrolysis acidification reactor showed obvious changes.; Provide a practical way to improve the efficiency of hydrolysis and acidification.; The role of Pseudomonas.;

Keywords

• cod
• hydrolysis acidification
• oily sewage
• pseudomonas stutzeri lh-42