Revista Brasileira de Parasitologia Veterinária (May 2021)

Platynosomum illiciens (Trematoda: Dicrocoeliidae) in a hybrid marmoset (Callithrix sp.) in the Municipality of Seropédica, RJ, Brazil – Case report

  • Rayane Christine Pereira de Assis,
  • Diefrey Ribeiro Campos,
  • Debora Azevedo Borges,
  • Barbara Rauta de Avelar,
  • Julia Aline Santos de Mello Pereira,
  • Carlos Alexandre Rey Matias,
  • Carlos Wilson Gomes Lopes,
  • Douglas McIntosh,
  • Fabio Barbour Scott

DOI
https://doi.org/10.1590/s1984-29612021012
Journal volume & issue
Vol. 30, no. 2

Abstract

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Abstract Platynosomum illiciens is a liver trematode encountered infecting mainly felids although it has also been reported in birds and in additional mammalian species, including non-human primates. The current study reports a natural P. illiciens infection primate of the genus Callithrix. The diagnosis was made using a combination of copro-parasitological techniques, morphological evaluation of adult specimens recovered from the liver during necropsy, and molecular analyses. Eggs were brown in color, oval, operculated, and contained a miracidium. Adult specimens recovered during necropsy were measured and showed dimensions compatible with P. illiciens. Molecular characterization of the trematode involved amplification by polymerase chain reaction (PCR), in combination with nucleotide sequencing, of an approximately 900 base pairs fragment corresponding to 18S-ITS1-5.8S ribosomal DNA. Sequenced amplicons showed 100% nucleotide identity with sequences deposited in the GenBank database as derived from specimens of P. illiciens recovered from cats in Malaysia and Brazil. It was concluded that the morphological and molecular analyses presented herein, confirmed the identification of the trematode recovered as P. illiciens.

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