Frontiers in Microbiology (Apr 2025)
Description of Pseudomonas imrae sp. nov., carrying a novel class C β-lactamase gene variant, isolated from gut samples of Atlantic mackerel (Scomber scombrus)
- Francisco Salvà-Serra,
- Francisco Salvà-Serra,
- Francisco Salvà-Serra,
- Francisco Salvà-Serra,
- Francisco Salvà-Serra,
- Priyank Nimje,
- Beatriz Piñeiro-Iglesias,
- Beatriz Piñeiro-Iglesias,
- Leonarda Achá Alarcón,
- Leonarda Achá Alarcón,
- Leonarda Achá Alarcón,
- Sofia Cardew,
- Sofia Cardew,
- Elisabeth Inganäs,
- Elisabeth Inganäs,
- Susanne Jensie-Markopoulos,
- Susanne Jensie-Markopoulos,
- Maria Ohlén,
- Maria Ohlén,
- Hanna-Sophia Sailer,
- Hanna-Sophia Sailer,
- Christel Unosson,
- Christel Unosson,
- Víctor Fernández-Juárez,
- Cesar O. Pacherres,
- Michael Kühl,
- Edward R. B. Moore,
- Edward R. B. Moore,
- Edward R. B. Moore,
- Edward R. B. Moore,
- Nachiket P. Marathe
Affiliations
- Francisco Salvà-Serra
- Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Francisco Salvà-Serra
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Francisco Salvà-Serra
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Francisco Salvà-Serra
- Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden
- Francisco Salvà-Serra
- Methodology Textiles and Medical Technology, Division Materials and Production, RISE Research Institutes of Sweden, Gothenburg, Sweden
- Priyank Nimje
- Institute of Marine Research (IMR), Bergen, Norway
- Beatriz Piñeiro-Iglesias
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Beatriz Piñeiro-Iglesias
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Leonarda Achá Alarcón
- Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Leonarda Achá Alarcón
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Leonarda Achá Alarcón
- Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden
- Sofia Cardew
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Sofia Cardew
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Elisabeth Inganäs
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Elisabeth Inganäs
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Susanne Jensie-Markopoulos
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Susanne Jensie-Markopoulos
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Maria Ohlén
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Maria Ohlén
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Hanna-Sophia Sailer
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Hanna-Sophia Sailer
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Christel Unosson
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Christel Unosson
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Víctor Fernández-Juárez
- Department of Biology and Nordic Center for Earth Evolution (NordCEE), University of Southern Denmark, Odense, Denmark
- Cesar O. Pacherres
- Marine Biological Section, Department of Biology, University of Copenhagen, Copenhagen, Denmark
- Michael Kühl
- Marine Biological Section, Department of Biology, University of Copenhagen, Copenhagen, Denmark
- Edward R. B. Moore
- Department of Infectious Diseases, Institute for Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Edward R. B. Moore
- Department of Clinical Microbiology, Sahlgrenska University Hospital, Gothenburg, Sweden
- Edward R. B. Moore
- Culture Collection University of Gothenburg (CCUG), Department of Clinical Microbiology, Sahlgrenska University Hospital and Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
- Edward R. B. Moore
- Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden
- Nachiket P. Marathe
- Institute of Marine Research (IMR), Bergen, Norway
- DOI
- https://doi.org/10.3389/fmicb.2025.1530878
- Journal volume & issue
-
Vol. 16
Abstract
Three β-lactam resistant bacterial strains isolated from gut samples of wild Atlantic mackerel (Scomber scombrus) collected from the northern North Sea were characterized by polyphasic analyses. The strains were determined to belong to the genus Pseudomonas but could not be assigned to a known species. The nearly-complete 16S rRNA gene sequence showed the highest similarity (99.9%) to four different species, although partial rpoD sequence exhibited relatively low similarities to Pseudomonas proteolytica (93.4%) and other Pseudomonas spp. Genome sequencing and subsequent digital DNA–DNA hybridization (dDDH), average nucleotide identity (ANI) analysis and core genome analysis confirmed that these strains represent a novel species within the genus Pseudomonas. The three strains demonstrated ANIb values >99.5% with each other, confirming that all three strains (CCUG 74779T = CECT 30571T, CCUG 74780 and CCUG 74781) belong to the same genomospecies. Phylogenomic analysis confirmed that the strains form a distinct genomic clade, representing a novel taxonomic species, for which the name Pseudomonas imrae sp. nov., is proposed, with strain CCUG 74779T (=CECT 30571T) designated as the type strain. We report the complete genome sequence of the type strain of P. imrae sp. nov. and show that it carries a gene encoding a novel variant of a chromosomally-encoded class C β-lactamase, which has been designated as PFL-7.
Keywords
- Pseudomonas fluorescens group
- Pseudomonas gessardii subgroup
- β-lactamase novel variant
- novel species
- polyphasic taxonomy
- phylogenomics
