مجلة مركز بحوث التقنيات الاحيائية (Jun 2015)

PCR Detection of Some ESBLs (bla) Genes in Pseudomonas aeruginosa Isolated from Burn’s Units in Bagdad Hospitals

  • Asmaa A. . AL-Kaisse,
  • Amina N. AL-Thwani,
  • Rabab Q. AL-Segar

DOI
https://doi.org/10.24126/jobrc.2015.9.2.439
Journal volume & issue
Vol. 9, no. 2

Abstract

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Multi drug resistant (MDR) and Extended Spectrum Beta Lactamase (ESBLs) of Pseudomonas aeruginosa were detected. Pseudomonas aeruginosa is a bacterium responsible for severe infections in burn’s units, plasmid DNA analysis and encoded many types of genes responsible for beta-lactamases. To determine the type of genes responsible for beta-lactam broad spectrum in P. aeruginosa strains isolated from 100 swabs of burn’s units environment, using a molecular methods (PCR) by primers specific to ESBLs (bla ) genes oxacillin hydrolyzing capabilities OXA-10, OXA-4 and Vietnam Extended-Spectrum β-Lactame VEB-1. The results revealed that 15 strains were isolated from burn units environment. All of 15 (100%) were positive OXA-10 and only one (6.6%) for OXA-4 while the other gene VEB-1 was found in 6 (40%) isolates.تم

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