Effect of androgen in peyronie plaque induction rats

Abstract

Objective Peyronie’s disease and hypogonadism are believed to have a linear association; however, research has yielded conflicting results. This is the first histopathological study on this topic.Methodology This study randomly assigned 40 three-month-old male Sprague-Dawley rats to five groups. We established Peyronie’s model by injecting intratunical TGF-beta and 3% disodium phosphate dihydrate into four groups. One group received intratunical saline. The control group for the Peyronie’s disease model was one group. In the other groups, the first had castration orchiectomy. The second group was the Peyronie’s disease model control. The third group received intratunical saline injections. The fourth group received intraperitoneal testosterone injections, while the fifth received 0.5 mg of 5-alpha reductase inhibitor orally daily. We excised penile tissue for histopathology after a 30-day follow-up. Masson’s trichrome staining found collagen and smooth muscle, picro-sirius staining found collagen III and collagen I, and hematoxylin and eosin staining found fibrosis.Results Histopathological examination revealed significant differences in fibrosis, smooth muscle/collagen ratios, and collagen III/collagen I ratios. Comparing groupings without a control group revealed no statistically significant differences.Conclusion This study explored testosterone’s effect on Peyronie’s disease in rats. Fibrosis was analyzed, but no significant changes were observed.

Keywords

• Peyronie’s disease
• rat model
• fibrosis
• transforming growth factor-beta
• testosterone