Human Tonsil-Derived Mesenchymal Stromal Cells Maintain Proliferating and ROS-Regulatory Properties via Stanniocalcin-1
Yoojin Seo,
Tae-Hoon Shin,
Ji-Su Ahn,
Su-Jeong Oh,
Ye Young Shin,
Ji Won Yang,
Hee Young Park,
Sung-Chan Shin,
Hyun-Keun Kwon,
Ji Min Kim,
Eui-Suk Sung,
Gi Cheol Park,
Byung-Joo Lee,
Hyung-Sik Kim
Affiliations
Yoojin Seo
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Tae-Hoon Shin
Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Ji-Su Ahn
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Su-Jeong Oh
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Ye Young Shin
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Ji Won Yang
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Hee Young Park
Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Sung-Chan Shin
Department of Otorhinolaryngology, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Hyun-Keun Kwon
Department of Otorhinolaryngology, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Ji Min Kim
Department of Otorhinolaryngology, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Eui-Suk Sung
Department of Otorhinolaryngology-Head and Neck Surgery, Biomedical Research Institute, Pusan National University School of Medicine, Yangsan Pusan National University Hospital, Yangsan 50612, Korea
Gi Cheol Park
Department of Otolaryngology – Head and Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon 51353, Korea
Byung-Joo Lee
Department of Otorhinolaryngology, College of Medicine, Pusan National University and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea
Hyung-Sik Kim
Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea
Mesenchymal stromal cells (MSCs) from various sources exhibit different potential for stemness and therapeutic abilities. Recently, we reported a unique MSCs from human palatine tonsil (TMSCs) and their superior proliferation capacity compared to MSCs from other sources. However, unique characteristics of each MSC are not yet precisely elucidated. We investigated the role of stanniocalcin-1 (STC1), an anti-oxidative hormone, in the functions of TMSCs. We found that STC1 was highly expressed in TMSC compared with MSCs from bone marrow or adipose tissue. The proliferation, senescence and differentiation of TMSCs were assessed after the inhibition of STC1 expression. STC1 inhibition resulted in a significant decrease in the proliferation of TMSCs and did not affect the differentiation potential. To reveal the anti-oxidative ability of STC1 in TMSCs themselves or against other cell types, the generation of mitochondrial reactive oxygen species (ROS) in TMSC or ROS-mediated production of interleukin (IL)-1β from macrophage-like cells were detected. Interestingly, the basal level of ROS generation in TMSCs was significantly elevated after STC1 inhibition. Moreover, down-regulation of STC1 impaired the inhibitory effect of TMSCs on IL-1β production in macrophages. Taken together, these findings indicate that STC1 is highly expressed in TMSCs and plays a critical role in proliferating and ROS-regulatory abilities.
