Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis

Jinhui Chen; Jinhui Chen; Mingming Liu; Mingming Liu; Xiangxu Meng; Xiangxu Meng; Yuanyuan Zhang; Yuanyuan Zhang; Yue Wang; Yue Wang; Nanbo Jiao; Nanbo Jiao; Jianmiao Chen; Jianmiao Chen

doi:10.3389/fpls.2023.1068796

Frontiers in Plant Science (Aug 2023)

Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis

Jinhui Chen,
Jinhui Chen,
Mingming Liu,
Mingming Liu,
Xiangxu Meng,
Xiangxu Meng,
Yuanyuan Zhang,
Yuanyuan Zhang,
Yue Wang,
Yue Wang,
Nanbo Jiao,
Nanbo Jiao,
Jianmiao Chen,
Jianmiao Chen

Affiliations

Jinhui Chen: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Jinhui Chen: Engineering Research Center of Rare and Precious Tree Species in Hainan Province, School of Forestry, Hainan University, Haikou, China
Mingming Liu: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Mingming Liu: School of Tropical Crops, Hainan University, Haikou, China
Xiangxu Meng: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Xiangxu Meng: Engineering Research Center of Rare and Precious Tree Species in Hainan Province, School of Forestry, Hainan University, Haikou, China
Yuanyuan Zhang: Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, China
Yuanyuan Zhang: State Centre for Rubber Breeding, Haikou, Hainan, China
Yue Wang: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Yue Wang: Engineering Research Center of Rare and Precious Tree Species in Hainan Province, School of Forestry, Hainan University, Haikou, China
Nanbo Jiao: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Nanbo Jiao: Engineering Research Center of Rare and Precious Tree Species in Hainan Province, School of Forestry, Hainan University, Haikou, China
Jianmiao Chen: Sanya Nanfan Research Institute of Hainan University, Hainan Yazhou Bay Seed Laboratory/Key Laboratory of Genetics and Germplasm Innovation of Tropical Special Forest Trees and Ornamental Plants, Ministry of Education, School of Forestry, Hainan University, Sanya, China
Jianmiao Chen: School of Tropical Crops, Hainan University, Haikou, China

DOI: https://doi.org/10.3389/fpls.2023.1068796
Journal volume & issue: Vol. 14

Abstract

Read online

IntroductionMicroRNAs (miRNAs) are small endogenous non-coding RNAs that play an important role in wood formation in plants. However, the significance of the link between miRNAs and their target transcripts in wood formation remains unclear in rubber tree (Hevea brasiliensis).MethodsIn this study, we induced the formation of reaction wood by artificially bending rubber trees for 300 days and performed small RNA sequencing and transcriptome deep sequencing (RNA-seq) to describe the complement of miRNAs and their targets contributing to this process.Results and discussionWe identified 5, 11, and 2 differentially abundant miRNAs in normal wood (NW) compared to tension wood (TW), in NW relative to opposite wood (OW), and between TW and OW, respectively. We also identified 12 novel miRNAs and 39 potential miRNA-mRNA pairs with different accumulation patterns in NW, TW, and OW. We noticed that many miRNAs targeted transcription factor genes, which were enriched in KEGG pathways associated with phenylpropanoid biosynthesis, phenylalanine metabolism, and pyruvate metabolism. Thus, miRNA-TF-mRNA network involved in wood formation via tension wood model were constructed. We validated the differential accumulation of miRNAs and their targets by RT-qPCR analysis and overexpressed miRNA in Nicotiana benthamiana with its potential target gene. These results will provide a reference for a deep exploration of growth and development in rubber tree.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

About the journal

Abstract

Keywords