Phytochemical characterization and multifaceted bioactivity assessment of essential oil from Ptychotis verticillata Duby: Anti-diabetic, anti-tyrosinase, and anti-inflammatory activity
Mohamed Taibi,
Amine Elbouzidi,
Mounir Haddou,
Abdellah Baraich,
El Hassania Loukili,
Tarik Moubchir,
Aimad Allali,
Amine khoulati,
Reda Bellaouchi,
Abdeslam Asehraou,
Mohamed Addi,
Ahmad Mohammad Salamatullah,
Mohammed Bourhia,
Farhan Siddique,
Bouchra El Guerrouj,
Khalid Chaabane
Affiliations
Mohamed Taibi
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco; Centre de L’Oriental des Sciences et Technologies de L’Eau et de L’Environnement (COSTEE), Université Mohammed Premier, Oujda, 60000, Morocco; Corresponding author. Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco.
Amine Elbouzidi
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco; Euro-Mediterranean University of Fes (UEMF), Fes, Morocco
Mounir Haddou
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco; Centre de L’Oriental des Sciences et Technologies de L’Eau et de L’Environnement (COSTEE), Université Mohammed Premier, Oujda, 60000, Morocco
Abdellah Baraich
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Boulevard Mohamed VI, B.P. 717, Oujda, 60000, Morocco
El Hassania Loukili
Euro-Mediterranean University of Fes (UEMF), Fes, Morocco
Tarik Moubchir
Polyvalent Team in Research and Development, Polydisciplinary Faculty of Beni Mellal (FPBM), University Sultan Moulay Slimane (USMS), Beni Mellal, 23000, Morocco
Aimad Allali
High Institute of Nursing Professions and Health Techniques Annex Taza, Fez, Morocco
Amine khoulati
Faculté de Médecine et de Pharmacie, Université Mohammed Premier, Oujda, 60000, Morocco
Reda Bellaouchi
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Boulevard Mohamed VI, B.P. 717, Oujda, 60000, Morocco
Abdeslam Asehraou
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Boulevard Mohamed VI, B.P. 717, Oujda, 60000, Morocco
Mohamed Addi
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco
Ahmad Mohammad Salamatullah
Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, 11 P.O. Box 2460, Riyadh, 11451, Saudi Arabia
Mohammed Bourhia
Laboratory of Biotechnology and Natural Resources Valorization, Faculty of Sciences, Ibn Zohr University, 80060, Agadir, Morocco; Corresponding author.
Farhan Siddique
Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, SE-60174, Norrköping, Sweden
Bouchra El Guerrouj
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco; Centre de L’Oriental des Sciences et Technologies de L’Eau et de L’Environnement (COSTEE), Université Mohammed Premier, Oujda, 60000, Morocco
Khalid Chaabane
Laboratoire d’Amélioration des Productions Agricoles, Biotechnologie et Environnement (LAPABE), Faculté des Sciences, Université Mohammed Premier, Oujda, 60000, Morocco
The aim of this study is to explore the pharmacological properties of the essential oil derived from Ptychotis verticillata Duby (PVEO), a medicinal plant native to Morocco, focusing on its antidiabetic, anti-tyrosinase, and anti-inflammatory effects. Additionally, the study aims to characterize the phytochemical composition of PVEO and evaluate its potential as a natural therapeutic alternative for various health conditions. To achieve this, phytochemical analysis was conducted using gas chromatography-mass spectrometry (GC-MS). Furthermore, in vitro assessments were conducted to investigate PVEO's antidiabetic activity by inhibiting α-amylase, xanthine oxidase, and α-glucosidase. Tests were also undertaken to evaluate the anti-inflammatory effect of PVEO on RAW 264.7 cells stimulated by lipopolysaccharide (LPS), as well as its efficacy as an anti-tyrosinase agent and its lipoxygenase inhibition activity. The results of the phytochemical analysis revealed that PVEO is rich in terpene compounds, with percentages of 40.35 % γ-terpinene, 22.40 % carvacrol, and 19.77 % β-cymene. Moreover, in vitro evaluations demonstrated that PVEO exhibits significant inhibitory activity against α-amylase, xanthine oxidase, and α-glucosidase, indicating promising antidiabetic, and anti-gout potential. Furthermore, PVEO showed significant anti-tyrosinase activity, with an IC50 of 27.39 ± 0.44 μg/mL, and remarkable lipoxygenase inhibition (87.33 ± 2.6 %), suggesting its candidacy for dermatoprotection. Additionally, PVEO displayed a dose-dependent capacity to attenuate the production of NO and PGE2, two inflammatory mediators implicated in various pathologies, without compromising cellular viability. The findings of this study provide a solid foundation for future research on natural therapies and the development of new drugs, highlighting the therapeutic potential of PVEO in the treatment of gout, diabetes, pigmentation disorders, and inflammation.
