Nature Communications (Jul 2017)
Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma
- Jianguo Huang,
- Mark Chen,
- Melodi Javid Whitley,
- Hsuan-Cheng Kuo,
- Eric S. Xu,
- Andrea Walens,
- Yvonne M. Mowery,
- David Van Mater,
- William C. Eward,
- Diana M. Cardona,
- Lixia Luo,
- Yan Ma,
- Omar M. Lopez,
- Christopher E. Nelson,
- Jacqueline N. Robinson-Hamm,
- Anupama Reddy,
- Sandeep S. Dave,
- Charles A. Gersbach,
- Rebecca D. Dodd,
- David G. Kirsch
Affiliations
- Jianguo Huang
- Department of Radiation Oncology, Duke University Medical Center
- Mark Chen
- Department of Pharmacology and Cancer Biology, Duke University Medical Center
- Melodi Javid Whitley
- Department of Pharmacology and Cancer Biology, Duke University Medical Center
- Hsuan-Cheng Kuo
- Department of Pharmacology and Cancer Biology, Duke University Medical Center
- Eric S. Xu
- Department of Radiation Oncology, Duke University Medical Center
- Andrea Walens
- Department of Pharmacology and Cancer Biology, Duke University Medical Center
- Yvonne M. Mowery
- Department of Radiation Oncology, Duke University Medical Center
- David Van Mater
- Division of Hematology-Oncology, Department of Pediatrics, Duke University Medical Center
- William C. Eward
- Department of Orthopedic Surgery, Duke University
- Diana M. Cardona
- Department of Pathology, Duke University
- Lixia Luo
- Department of Radiation Oncology, Duke University Medical Center
- Yan Ma
- Department of Radiation Oncology, Duke University Medical Center
- Omar M. Lopez
- Department of Pharmacology and Cancer Biology, Duke University Medical Center
- Christopher E. Nelson
- Department of Biomedical Engineering, Duke University
- Jacqueline N. Robinson-Hamm
- Department of Biomedical Engineering, Duke University
- Anupama Reddy
- Duke Center for Genomic and Computational Biology, Duke University
- Sandeep S. Dave
- Duke Center for Genomic and Computational Biology, Duke University
- Charles A. Gersbach
- Department of Biomedical Engineering, Duke University
- Rebecca D. Dodd
- Department of Radiation Oncology, Duke University Medical Center
- David G. Kirsch
- Department of Radiation Oncology, Duke University Medical Center
- DOI
- https://doi.org/10.1038/ncomms15999
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 11
Abstract
Site-specific recombination and CRISPR-Cas9 have been used to generate genetically engineered mouse models of cancer. Here the authors compare sarcomas generated using both systems and see similar genetic and cellular phenotypes, suggesting CRISPR-Cas9 can be used to rapidly generate sarcoma models.
