Two distinct modes of action of molecular glues in the plant hormone co-receptor COI1-JAZ system
Takuya Kaji,
Kotaro Matsumoto,
Taichi Okumura,
Misuzu Nakayama,
Shunji Hoshino,
Yousuke Takaoka,
Jianxin Wang,
Minoru Ueda
Affiliations
Takuya Kaji
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Kotaro Matsumoto
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Taichi Okumura
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Misuzu Nakayama
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Shunji Hoshino
Department of Molecular and Chemical Life Sciences, Graduate School of Life Science, Tohoku University, Sendai 980-8578, Japan
Yousuke Takaoka
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Jianxin Wang
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan
Minoru Ueda
Department of Chemistry, Graduate School of Science, Tohoku University, Sendai 980-8578, Japan; Department of Molecular and Chemical Life Sciences, Graduate School of Life Science, Tohoku University, Sendai 980-8578, Japan; Corresponding author
Summary: The plant hormone (3R, 7S)-jasmonoyl-L-isoleucine ((3R, 7S)-JA-Ile) is perceived by the COI1-JAZ co-receptor in Arabidopsis thaliana, leading to the activation of gene expression for plant defense responses, growth, development, and other processes. Therefore, understanding the interaction between the COI1-JAZ co-receptor and its ligands is essential for the development of COI1-JAZ agonists and antagonists as potent chemical tools for regulating (3R, 7S)-JA-Ile signaling. This study demonstrated that COI1-JAZ has two independent modes of ligand perception using a differential scanning fluorimetry (DSF) assay. (3R, 7S)-JA-Ile is perceived through a one-step model in which (3R, 7S)-JA-Ile causes protein–protein interaction between COI1 and JAZ. In contrast, coronatine (COR), a mimic of (3R, 7S)-JA-Ile, is perceived through a two-step model in which COR is first perceived by COI1 and then recruits JAZ to form the COI1-COR-JAZ complex. Our results demonstrate two distinct modes of action of molecular glues causing protein–protein interactions.
