A study on the mechanism of the protective effect of guangefang on sepsis-associated acute kidney injury

Abstract

Objective: The objective of this study was to explore the mechanism of Guan Gefang (GGF) ; raw rhubarb 30 g, cassia arboreal 30 g, raw oyster 30 g, ground elm 60 g, and dandelion 30 g) kidney protection. Materials and Methods: Thirty-six Sprague Dawley rats were randomly divided into a control group (Group N), a sepsis control group (Group S), and a sepsis + GGF group (Group G). For Group N, 8 ml/kg 0.9% NaCl was used as an enema; for Group S, cecal ligation and puncture (CLP) method was used for modeling and 8 ml/kg 0.9% NaCl was used as an enema; and Group G, CLP was used for modeling and 8 ml/kg GGF was used as an enema. All of the enemas were applied once daily for 4 days. The indices of serum creatinine (SCr), blood urea nitrogen (BUN), uric acid (UA), mammalian target of rapamycin (mTOR), Janus kinase 2 (JAK2) were compared across each group. Results: Compared to Group S, Group G had lower levels of SCr, BUN, and UA (P < 0.05), while the activities of mTOR and JAK2 were significantly inhibited. Conclusion: GGF may have inhibited the JAK2 or mTOR signaling pathways to protect the rats' kidneys, which had sepsis-associated acute kidney injury.

Keywords

• guangefang
• janus kinase 2
• mammalian target of rapamycin
• sepsis-associated acute kidney injury