Frontiers in Neuroscience (Jul 2018)

Silencing miR-150 Ameliorates Experimental Autoimmune Encephalomyelitis

  • Zhaolan Hu,
  • Yanhui Cui,
  • Xiaoqing Qiao,
  • Xinwen He,
  • Fang Li,
  • Cong Luo,
  • Shuang Wang,
  • Changqi Li,
  • Ruping Dai

DOI
https://doi.org/10.3389/fnins.2018.00465
Journal volume & issue
Vol. 12

Abstract

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MiR-150 regulates maturation and differentiation of T cells but how it functions in multiple sclerosis (MS) is unclear. In miR-150 knockout (KO) mice, we examined the effect of miR-150 deletion on disease severity of experimental autoimmune encephalomyelitis (EAE), an animal model of MS. After deleting miR-150, EAE disease severity was reduced according to clinical score. Histological staining and MBP immunofluorescence staining revealed that miR-150 deletion limited the extent of inflammatory demyelination and axonal damage in the spinal cord. Flow cytometry showed that CD3+, CD4+, and CD8+ T cells were increased in WT-EAE mice, but miR-150 deletion significantly reversed EAE-mediated up-regulation of CD3+, CD4+, and CD8+ T cells and down-regulation of CD19+ B cells. In addition, miR-150 deletion reduced the mRNA expression of IL-1β, IL-6, IL-17, and TNF-α in spleen and spinal cord after EAE induction. Thus, miR-150 deletion reduces EAE severity and demyelination, probably through inhibiting the activated immune response and the inflammation in the central nervous system.

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