PCR Differentiation of <i>Francisella tularensis</i> Subspecies Using One Primer

Abstract

Presented are experimental data of size distribution of amplicons obtained with the help of the primer consisting of Escherichia coli chi-sequence and DNA from different subspecies of Francisella tularensis strains. Analysis of this distribution permitted to determine five informative regions on the electrophoregrams (in the regions of 190, 280, 500-570, 830 and 950 b.p.), that made it possible to perform subspecies differentiation of tularemia microbe strains. Thus, the subspecies of F. tularensis clinical isolates can be identified by safe, fast and convenient method - the PCR analysis using only one primer Chi 1f despite the slight genetic heterogeneity of tularemia agent strains.

Keywords

• <i>f. tularensis</i>
• subspecies
• pcr
• typing
• primer