SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation

Jingting Li; Xiaojun Xu; Manisha Tiwari; Yifang Chen; Mackenzie Fuller; Varun Bansal; Pablo Tamayo; Soumita Das; Pradipta Ghosh; George L. Sen

doi:10.1038/s41467-021-21067-w

Nature Communications (Feb 2021)

SPT6 promotes epidermal differentiation and blockade of an intestinal-like phenotype through control of transcriptional elongation

Jingting Li,
Xiaojun Xu,
Manisha Tiwari,
Yifang Chen,
Mackenzie Fuller,
Varun Bansal,
Pablo Tamayo,
Soumita Das,
Pradipta Ghosh,
George L. Sen

Affiliations

Jingting Li: Department of Dermatology, Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego
Xiaojun Xu: Moores Cancer Center, University of California, San Diego
Manisha Tiwari: Department of Dermatology, Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego
Yifang Chen: Department of Dermatology, Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego
Mackenzie Fuller: Departments of Medicine and Cellular and Molecular Medicine, HUMANOID Center of Research Excellence, University of California, San Diego
Varun Bansal: Department of Dermatology, Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego
Pablo Tamayo: Moores Cancer Center, University of California, San Diego
Soumita Das: Department of Pathology, HUMANOID Center of Research Excellence, University of California, San Diego
Pradipta Ghosh: Departments of Medicine and Cellular and Molecular Medicine, HUMANOID Center of Research Excellence, University of California, San Diego
George L. Sen: Department of Dermatology, Department of Cellular and Molecular Medicine, UCSD Stem Cell Program, University of California, San Diego

DOI: https://doi.org/10.1038/s41467-021-21067-w
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 15

Abstract

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The role of RNA polymerase II (Pol II) recruitment during adult stem cell differentiation is well understood, but not that of Pol II elongation. Here, the authors show that 30% of epidermal stem cell differentiation genes depend on SPT6 and PAF1 for Pol II elongation, and SPT6 loss leads to an intestine-like phenotype.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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