Profiling surface proteins on individual exosomes using a proximity barcoding assay

Di Wu; Junhong Yan; Xia Shen; Yu Sun; Måns Thulin; Yanling Cai; Lotta Wik; Qiujin Shen; Johan Oelrich; Xiaoyan Qian; K. Louise Dubois; K. Göran Ronquist; Mats Nilsson; Ulf Landegren; Masood Kamali-Moghaddam

doi:10.1038/s41467-019-11486-1

Nature Communications (Aug 2019)

Profiling surface proteins on individual exosomes using a proximity barcoding assay

Di Wu,
Junhong Yan,
Xia Shen,
Yu Sun,
Måns Thulin,
Yanling Cai,
Lotta Wik,
Qiujin Shen,
Johan Oelrich,
Xiaoyan Qian,
K. Louise Dubois,
K. Göran Ronquist,
Mats Nilsson,
Ulf Landegren,
Masood Kamali-Moghaddam

Affiliations

Di Wu: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Junhong Yan: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Xia Shen: Center for Global Health Research, Usher Institute of Population Health Sciences and Informatics, University of Edinburgh
Yu Sun: Department of Molecular Evolution, Uppsala University
Måns Thulin: Department of Statistics, Uppsala University
Yanling Cai: Institute of Translational Medicine, The Second Peopleʹs Hospital of Shenzhen
Lotta Wik: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Qiujin Shen: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Johan Oelrich: Vesicode AB
Xiaoyan Qian: Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University
K. Louise Dubois: Department of Medical Sciences, Clinical Chemistry, Uppsala University
K. Göran Ronquist: Department of Medical Sciences, Clinical Chemistry, Uppsala University
Mats Nilsson: Department of Biochemistry and Biophysics, Science for Life Laboratory, Stockholm University
Ulf Landegren: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University
Masood Kamali-Moghaddam: Department of Immunology, Genetics and Pathology, Science for Life Laboratory, Uppsala University

DOI: https://doi.org/10.1038/s41467-019-11486-1
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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The use of antibodies to capture and profile exosomes limits the number of target proteins that can be detected. Here the authors develop a proximity-dependent barcoding assay that allows profiling of 38 surface proteins on individual exosomes from heterogeneous samples such as serum and seminal fluid.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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