Profiling of Potential Antibacterial Compounds of Lactic Acid Bacteria against Extremely Drug Resistant (XDR) <i>Acinetobacter baumannii</i>
Phui-Chyng Yap,
Noorfazlin Ayuhan,
Jia Jie Woon,
Cindy Shuan Ju Teh,
Vannajan Sanghiran Lee,
Adzzie Shazleen Azman,
Sazaly AbuBakar,
Hai Yen Lee
Affiliations
Phui-Chyng Yap
Tropical Infectious Diseases Research and Education Center (TIDREC), Higher Institution Center of Excellence (HiCOE), Universiti Malaya, Kuala Lumpur 50603, Malaysia
Noorfazlin Ayuhan
Tropical Infectious Diseases Research and Education Center (TIDREC), Higher Institution Center of Excellence (HiCOE), Universiti Malaya, Kuala Lumpur 50603, Malaysia
Jia Jie Woon
Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia
Cindy Shuan Ju Teh
Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur 50603, Malaysia
Vannajan Sanghiran Lee
Department of Chemistry, Center of Theoretical and Computational Physics (CTCP), Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia
Adzzie Shazleen Azman
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway 47500, Malaysia
Sazaly AbuBakar
Tropical Infectious Diseases Research and Education Center (TIDREC), Higher Institution Center of Excellence (HiCOE), Universiti Malaya, Kuala Lumpur 50603, Malaysia
Hai Yen Lee
Tropical Infectious Diseases Research and Education Center (TIDREC), Higher Institution Center of Excellence (HiCOE), Universiti Malaya, Kuala Lumpur 50603, Malaysia
A total of 20 of isolates of lactic acid bacteria (LAB) were selected and screened for antagonistic activity against clinical strains of 30 clinical isolates of extremely drug-resistant (XDR) Acinetobacter baumannii using the well diffusion assay method. Results showed that 50% of the highly LAB strains possessed inhibitory activity against (up to 66%) of the XDR A. baumannii strains tested. The supernatant of the twenty LAB strains was subjected to gas chromatography mass spectrometry (GCMS) revealed that the common compound found in the active isolates against XDR A. baumannii was 3-Isobutyl-2,3,6,7,8,8a-hexahydropyrrolo[1,2-a]pyrazine-1,4-dione, a known potential diketopiperazine group. The molecular docking study against potential antibacterial targets with selected ligands was performed to predict the binding mode of interactions, which is responsible for antibacterial activity. The docking analysis of the potent compounds supported the potential antibacterial activity exhibiting high inhibition constant and binding affinity in silico.
