Generation of induced pluripotent stem cell lines (RFSCi003-A, RFSCi004-A) from monozygotic twins discordant for age-related macular degeneration

Naresh Rajendran; Wendy Runyon; Sam Hu; Ajeet Singh; Rinki Ratnapriya; Ritu Kumar; Karl Csaky; Srinivasa R. Sripathi

doi:10.1016/j.scr.2025.103768

Stem Cell Research (Sep 2025)

Generation of induced pluripotent stem cell lines (RFSCi003-A, RFSCi004-A) from monozygotic twins discordant for age-related macular degeneration

Naresh Rajendran,
Wendy Runyon,
Sam Hu,
Ajeet Singh,
Rinki Ratnapriya,
Ritu Kumar,
Karl Csaky,
Srinivasa R. Sripathi

Affiliations

Naresh Rajendran: Henderson Ocular Stem Cell Laboratory, Retina Foundation of the Southwest, Dallas, TX 75231, USA
Wendy Runyon: Stem Cell Core, Gladstone Institutes, San Francisco, CA 94158, USA
Sam Hu: Stem Cell Core, Gladstone Institutes, San Francisco, CA 94158, USA
Ajeet Singh: Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
Rinki Ratnapriya: Department of Ophthalmology, Baylor College of Medicine, Houston, TX 77030, USA
Ritu Kumar: Stem Cell Core, Gladstone Institutes, San Francisco, CA 94158, USA
Karl Csaky: Molecular Ophthalmology Laboratory, Clinical Center of Innovation for AMD, Retina Foundation of the Southwest, Dallas, TX 75231, USA; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
Srinivasa R. Sripathi: Henderson Ocular Stem Cell Laboratory, Retina Foundation of the Southwest, Dallas, TX 75231, USA; Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Corresponding author at: Henderson Ocular Stem Cell Laboratory, Retina Foundation of the Southwest, Dallas, TX 75231, USA.

DOI: https://doi.org/10.1016/j.scr.2025.103768
Journal volume & issue: Vol. 87
p. 103768

Abstract

Read online

Age-related macular degeneration (AMD) has significant genetic component, yet monozygotic twins frequently exhibit discordance in disease status, highlighting the role of non-genetic factors. To enable comparative studies of AMD pathogenesis, we generated two induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMCs) of a monozygotic twin pair discordant for AMD. These iPSC lines offer a unique genetically matched resource to investigate molecular differences between affected and unaffected twins, as well as their responses to genetic, epigenetic, and environmental contributors to AMD development.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.sciencedirect.com/journal/stem-cell-research

About the journal