Nature Communications (Mar 2020)

Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps

  • Jun Yao,
  • Yang He,
  • Nannan Su,
  • Sakshibeedu R. Bharath,
  • Yong Tao,
  • Jian-Ming Jin,
  • Wei Chen,
  • Haiwei Song,
  • Shuang-Yan Tang

DOI
https://doi.org/10.1038/s41467-020-14918-5
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Whole-cell catalyst-based hydroxytyrosol production is low. Here, the authors increase the efficiency of its production in E. coli by de-bottlenecking two enzymatic steps catalyzed by monooxygenase and tyramine oxidase using structure-based enzyme redesign or in vivo-directed evolution with the aid of a newly developed biosensor.