Journal of Fungi (Jun 2024)

Differential Carbon Catabolite Repression and Hemicellulolytic Ability among Pathotypes of <i>Colletotrichum lindemuthianum</i> against Natural Plant Substrates

  • Karla Morelia Díaz-Tapia,
  • María Guadalupe Zavala-Páramo,
  • Maria Guadalupe Villa-Rivera,
  • Ma. Irene Morelos-Martínez,
  • Everardo López-Romero,
  • June Simpson,
  • Jeni Bolaños-Rebolledo,
  • Horacio Cano-Camacho

DOI
https://doi.org/10.3390/jof10060406
Journal volume & issue
Vol. 10, no. 6
p. 406

Abstract

Read online

Colletotrichum lindemuthianum is a phytopathogenic fungus that causes anthracnose in common beans (Phaseolus vulgaris) and presents a great diversity of pathotypes with different levels of virulence against bean varieties worldwide. The purpose of this study was to establish whether pathotypic diversity is associated with differences in the mycelial growth and secretion of plant-cell-wall-degrading enzymes (PCWDEs). We evaluated growth, hemicellulase and cellulase activity, and PCWDE secretion in four pathotypes of C. lindemuthianum in cultures with glucose, bean hypocotyls and green beans of P. vulgaris, and water hyacinth (Eichhornia crassipes). The results showed differences in the mycelial growth, hemicellulolytic activity, and PCWDE secretion among the pathotypes. Glucose was not the preferred carbon source for the best mycelial growth in all pathotypes, each of which showed a unique PCWDE secretion profile, indicating different levels of carbon catabolite regulation (CCR). The pathotypes showed a high differential hemicellulolytic capacity to degrade host and water hyacinth tissues, suggesting CCR by pentoses and that there are differences in the absorption and metabolism of different monosaccharides and/or disaccharides. We propose that different levels of CCR could optimize growth in different host tissues and could allow for consortium behavior in interactions with bean crops.

Keywords