Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer

Koji Murono; Soichiro Ishihara; Kazushige Kawai; Manabu Kaneko; Kazuhito Sasaki; Kensuke Otani; Koji Yasuda; Takeshi Nishikawa; Toshiaki Tanaka; Tomomichi Kiyomatsu; Keisuke Hata; Hiroaki Nozawa; Yumiko Satoh; Makiko Kurihara; Yutaka Yatomi; Toshiaki Watanabe

Asian Journal of Surgery (Jul 2018)

Significance of carcinoembryonic antigen mRNA in peritoneal lavage determined by transcription–reverse transcription concerted method in patients with low rectal cancer

Koji Murono,
Soichiro Ishihara,
Kazushige Kawai,
Manabu Kaneko,
Kazuhito Sasaki,
Kensuke Otani,
Koji Yasuda,
Takeshi Nishikawa,
Toshiaki Tanaka,
Tomomichi Kiyomatsu,
Keisuke Hata,
Hiroaki Nozawa,
Yumiko Satoh,
Makiko Kurihara,
Yutaka Yatomi,
Toshiaki Watanabe

Affiliations

Koji Murono: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan; Corresponding author. Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
Soichiro Ishihara: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Kazushige Kawai: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Manabu Kaneko: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Kazuhito Sasaki: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Kensuke Otani: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Koji Yasuda: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Takeshi Nishikawa: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Toshiaki Tanaka: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Tomomichi Kiyomatsu: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Keisuke Hata: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Hiroaki Nozawa: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Yumiko Satoh: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Makiko Kurihara: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Yutaka Yatomi: Department of Clinical Laboratory, Faculty of Medicine, University of Tokyo, Tokyo, Japan
Toshiaki Watanabe: Division of Surgical Oncology, Department of Surgery, Faculty of Medicine, University of Tokyo, Tokyo, Japan

Journal volume & issue: Vol. 41, no. 4
pp. 321 – 327

Abstract

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Summary: Background/Objective: Positive carcinoembryonic antigen (CEA) messenger RNA (mRNA) in peritoneal lavage is associated with poor prognosis in patients with colon cancer. However, there are no reports about rectal cancer. Therefore we aimed to evaluate the frequency of positive CEA mRNA in peritoneal lavage and the significance of CEA mRNA in patients with low rectal cancer. Methods: A total of 55 patients with low rectal cancer who received curative surgical resection were enrolled. CEA mRNA in peritoneal lavage was measured using the transcription–reverse transcription concerted method, a quantitative RNA amplification method. The correlation between CEA mRNA and overall and peritoneal recurrence-free survival was evaluated. Results: Among 55 patients, 6 (10.9%) had positive CEA mRNA in peritoneal lavage. Patients with positive CEA mRNA resulted in significantly higher recurrence rate than those with negative CEA mRNA (p=0.007). Similarly, the local recurrence rate was significantly higher in the positive CEA mRNA group than in the negative CEA mRNA group (p=0.0009). Lymph node metastasis and positive CEA mRNA were independent risk factors for overall and local recurrence. Conclusion: Positive CEA mRNA in low rectal cancer is a factor that predisposes patients to a high risk for overall recurrence, especially for local recurrence. Keywords: carcinoembryonic antigen messenger RNA, low rectal cancer, micrometastasis, peritoneal lavage, transcription–reverse transcription concerted method

Published in Asian Journal of Surgery

ISSN: 1015-9584 (Print)
Publisher: Elsevier
Country of publisher: Hong Kong
LCC subjects: Medicine: Surgery
Website: http://www.journals.elsevier.com/asian-journal-of-surgery/

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