Polymorphism and differential expression of MHC classⅠgenes between different strains of guinea pig

Abstract

Guinea pig is a laboratory animal model to study the illness and stimulation of infection. The previous studies showed that the sensitivity of Zmu-1∶DHP outbred guinea pig to foot-and-mouth disease virus was 100%, which was significantly different from DHP (parental strain). This study took three strains of guinea pigs (Zmu-1∶DHP outbred line, Zmu-2∶DHP outbred line and DHP strain) as the experimental groups. The RNA extracted from spleen of different strains of guinea pigs was transcribed into cDNA and amplified by polymerase chain reaction. The amplified products were sequenced by high-throughput sequencing to analyze the structure, haplotype and polymorphism of MHC classⅠgene in guinea pigs, so as to detect whether there were differences in the expression of MHC classⅠgene among different strains. The results showed that eight MHC classⅠsequences in guinea pig were found and some of these haplotypes had 23 amino acids deletion in exon 3. There were significant differences in the frequencies of haplotype expression reads among some strains (P＜0.05), which suggested that the difference of MHC classⅠhaplotype expression might be related to the difference of immune ability among different strains. The research provides a theoretical basis for the application of guinea pig strains in disease model and vaccine development.

Keywords

• guinea pig
• Zmu strain
• MHC classⅠgene