Association between combinations of genetic polymorphisms and epidemiopathogenic forms of bovine paratuberculosis
Ramon A. Juste,
Patricia Vazquez,
Otsanda Ruiz-Larrañaga,
Mikel Iriondo,
Carmen Manzano,
Mikel Agirre,
Andone Estonba,
Maria V. Geijo,
Elena Molina,
Iker A. Sevilla,
Marta Alonso-Hearn,
Nieves Gomez,
Valentin Perez,
Adoracion Cortes,
Joseba M. Garrido
Affiliations
Ramon A. Juste
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Patricia Vazquez
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Otsanda Ruiz-Larrañaga
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Bizkaia, Spain
Mikel Iriondo
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Bizkaia, Spain
Carmen Manzano
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Bizkaia, Spain
Mikel Agirre
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Bizkaia, Spain
Andone Estonba
Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), 48940 Leioa, Bizkaia, Spain
Maria V. Geijo
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Elena Molina
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Iker A. Sevilla
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Marta Alonso-Hearn
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Nieves Gomez
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Valentin Perez
Animal Health Department, Faculty of Veterinary Sciences, University of León, Campus de Vegazana s/n, 24071 León, Spain
Adoracion Cortes
Department of Health, Basque Government, Matadero Frigorífico Donostiarra, S.A.L. (MAFRIDO), P° Ubarburu 16, 20014 Donostia-San Sebastian, Spain
Joseba M. Garrido
Animal Health Department, NEIKER-Tecnalia, Berreaga, 1, 48160 Derio, Bizkaia, Spain
Control of major mycobacterial diseases affecting livestock is a challenging issue that requires different approaches. The use of genetic markers for improving resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle has been explored as a promising population strategy We performed paratuberculosis epidemiopathogenic phenotypic and genotypic characterization involving 24 SNPs in six candidate genes (NOD2, CD209, SLC11A1, SP110, TLR2 and TLR4) on 502 slaughtered Friesian cows. In the current study, we investigate whether recently proposed paratuberculosis (PTB) epidemiopathogenic (EP) forms (apparently free-AF, latent-LAT and patent-PAT) could be associated with some combination of these 24 SNPs. Best EP form grouping was obtained using a combination of 5 SNPs in four genes (CD209: rs210748127; SLC11A1: rs110090506; SP110: rs136859213 and rs110480812; and TLR2: rs41830058). These groups were defined according to the level of infection progression risk to patent epidemiopathogenic forms and showed the following distributions: LOWIN (low) with 39 (8%) cases (94.9% AF/5.1% LAT/0% PAT); LATIN (low) with 17 (3%) cases (5.9% AF/94.1% LAT/0% PAT); AVERIN (average) with 413 (82%) cases (52.1% AF/38.5% LAT/9.4% PAT) and PATIN (patent) with 33 (7%) cases (36.4% AF/24.2% LAT/39.4% PAT). Age of slaughter was significantly higher for LATIN (88.3 months) compared to AVERIN (65.3 months; p = 0.0007) and PATIN (59.1 months; p = 0.0004), and for LOWIN (73.9 months) compared to PATIN (p = 0.0233), and nearly significant compared to AVERIN (p = 0.0572) These results suggest that some selected genetic polymorphisms have a potential use as markers of PTB EP forms and thus add a new tool for the control of this widespread infection.
