Nature Communications (Aug 2017)
Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry
- Ben C. Collins,
- Christie L. Hunter,
- Yansheng Liu,
- Birgit Schilling,
- George Rosenberger,
- Samuel L. Bader,
- Daniel W. Chan,
- Bradford W. Gibson,
- Anne-Claude Gingras,
- Jason M. Held,
- Mio Hirayama-Kurogi,
- Guixue Hou,
- Christoph Krisp,
- Brett Larsen,
- Liang Lin,
- Siqi Liu,
- Mark P. Molloy,
- Robert L. Moritz,
- Sumio Ohtsuki,
- Ralph Schlapbach,
- Nathalie Selevsek,
- Stefani N. Thomas,
- Shin-Cheng Tzeng,
- Hui Zhang,
- Ruedi Aebersold
Affiliations
- Ben C. Collins
- Department of Biology, Institute of Molecular Systems Biology
- Christie L. Hunter
- SCIEX
- Yansheng Liu
- Department of Biology, Institute of Molecular Systems Biology
- Birgit Schilling
- Buck Institute for Research on Aging
- George Rosenberger
- Department of Biology, Institute of Molecular Systems Biology
- Samuel L. Bader
- Institute for Systems Biology
- Daniel W. Chan
- Department of Pathology, Clinical Chemistry Division, Johns Hopkins University School of Medicine
- Bradford W. Gibson
- Buck Institute for Research on Aging
- Anne-Claude Gingras
- Lunenfeld-Tanenbaum Research Institute, Sinai Health System
- Jason M. Held
- Departments of Medicine and Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue
- Mio Hirayama-Kurogi
- Department of Pharmaceutical Microbiology, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi
- Guixue Hou
- Proteomics Division, BGI-Shenzhen
- Christoph Krisp
- Department of Chemistry and Biomolecular Sciences, Australian Proteome Analysis Facility (APAF), Macquarie University
- Brett Larsen
- Lunenfeld-Tanenbaum Research Institute, Sinai Health System
- Liang Lin
- Proteomics Division, BGI-Shenzhen
- Siqi Liu
- Proteomics Division, BGI-Shenzhen
- Mark P. Molloy
- Department of Chemistry and Biomolecular Sciences, Australian Proteome Analysis Facility (APAF), Macquarie University
- Robert L. Moritz
- Institute for Systems Biology
- Sumio Ohtsuki
- Department of Pharmaceutical Microbiology, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi
- Ralph Schlapbach
- Functional Genomics Center Zurich, ETH Zurich/University of Zurich
- Nathalie Selevsek
- Functional Genomics Center Zurich, ETH Zurich/University of Zurich
- Stefani N. Thomas
- Department of Pathology, Clinical Chemistry Division, Johns Hopkins University School of Medicine
- Shin-Cheng Tzeng
- Departments of Medicine and Anesthesiology, Washington University School of Medicine, 660 South Euclid Avenue
- Hui Zhang
- Department of Pathology, Clinical Chemistry Division, Johns Hopkins University School of Medicine
- Ruedi Aebersold
- Department of Biology, Institute of Molecular Systems Biology
- DOI
- https://doi.org/10.1038/s41467-017-00249-5
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 12
Abstract
SWATH-mass spectrometry consists of a data-independent acquisition and a targeted data analysis strategy that aims to maintain the favorable quantitative characteristics on the scale of thousands of proteins. Here, using data generated by eleven groups worldwide, the authors show that SWATH-MS is capable of generating highly reproducible data across different laboratories.
