Notulae Scientia Biologicae (Nov 2011)

Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron

  • Panjan SUJJARITTHURAKARN,
  • Kamnoon KANCHANAPOOM

Journal volume & issue
Vol. 3, no. 4
pp. 88 – 92

Abstract

Read online

An efficient induction of protocorm-like bodies (PLBs) from seedlings of dwarf Dendrobium was accomplished in order to develop mass-scale propagation. The effect of N6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro induction of PLBs was studied. TDZ was found to be a more effective inducer of PLBs and their proliferation than BA. The highest percentage for the induction of PLBs (86%) and the highest number of PLBs (3.6) per protocorm were observed after 9 weeks of culture in modified Murashige and Skoog (MS) liquid medium supplemented with 18 ?M TDZ.