Bio-Protocol (May 2021)

Parasitemia Evaluation in Mice Infected with Schistosoma mansoni

  • Naiara Tavares,
  • Marina Mourão

DOI
https://doi.org/10.21769/bioprotoc.4017
Journal volume & issue
Vol. 11, no. 10

Abstract

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Schistosomiasis is a neglected tropical disease. Its treatment relies on the use of a single drug, praziquantel. Due to treatment limitations, an alternative for schistosomiasis chemotherapy is required; thus, a better understanding of parasite biology and host-parasite interactions is valuable to aid the identification of new anti-Schistosoma drugs. The parasite has a complex life cycle, which results in challenges regarding the evaluation of Schistosoma mansoni development and mammalian infection establishment. Accordingly, this protocol describes methodologies to evaluate: (1) adult worm growth; (2) reproduction; and (3) granuloma formation; and consequently allows more comprehensive knowledge of S. mansoni development in a natural biological system.