Nature Communications (Sep 2016)

Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

  • Ranhua Xiong,
  • Roosmarijn E. Vandenbroucke,
  • Katleen Broos,
  • Toon Brans,
  • Elien Van Wonterghem,
  • Claude Libert,
  • Jo Demeester,
  • Stefaan C. De Smedt,
  • Kevin Braeckmans

DOI
https://doi.org/10.1038/ncomms12982
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

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Measuring the size distribution of nanomaterials in biological fluids is crucial to understand their properties in vivo. Here, the authors apply fluorescence recovery after photobleaching to measure protein aggregation in serum and to study permeability of biological membranes in mouse models.