Nature Communications (Feb 2020)

Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

  • Dorte B. Bekker-Jensen,
  • Oliver M. Bernhardt,
  • Alexander Hogrebe,
  • Ana Martinez-Val,
  • Lynn Verbeke,
  • Tejas Gandhi,
  • Christian D. Kelstrup,
  • Lukas Reiter,
  • Jesper V. Olsen

DOI
https://doi.org/10.1038/s41467-020-14609-1
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 12

Abstract

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Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.