Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

Tetsuya Mori; Shogo Sugiyama; Mark Byrne; Carl Hirschie Johnson; Takayuki Uchihashi; Toshio Ando

doi:10.1038/s41467-018-05438-4

Nature Communications (Aug 2018)

Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

Tetsuya Mori,
Shogo Sugiyama,
Mark Byrne,
Carl Hirschie Johnson,
Takayuki Uchihashi,
Toshio Ando

Affiliations

Tetsuya Mori: Department of Biological Sciences, Vanderbilt University
Shogo Sugiyama: Department of Physics, College of Science and Engineering, Kanazawa University
Mark Byrne: Department of Chemistry, Physics, and Engineering
Carl Hirschie Johnson: Department of Biological Sciences, Vanderbilt University
Takayuki Uchihashi: Department of Physics and Structural Biology Research Center, Nagoya University
Toshio Ando: Nano Life Science Institute (WPI-NanoLSI), Kanazawa University

DOI: https://doi.org/10.1038/s41467-018-05438-4
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 13

Abstract

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The circadian clock proteins KaiA, KaiB, and KaiC reconstitute a circa-24 h oscillation of KaiC phosphorylation in vitro. Here the authors use high-speed atomic force microscopy to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on the sub-second timescale to discover mechanisms of oscillatory resilience.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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