PRIMARY ISOLATION OF NEWCASTLE DISEASE VIRUS IN MDBK CELLINE

Abstract

Newcastle disease is a dreadful disease of poultry but accurate diagnosis of the disease almost always involves the isolation and characterisation of the virus (Alexander, 1988). For isolation of Newcastle disease virus (NDV) embryonated hens eggs (Beard and Hanson, 1981) and various cell lines like BHK-21 (French and George, 1965), Mouse L - 929 (Alexander et al., 1973 a) MDBK (Alexander et al., 1973 b; King, 1993) Pig kidney (Jully and Hipplito, 1973; Bansal and Kumar, 1975), she ep kidney (Ponkova et al.,1972) and QT-35 (Reddy and Srinivasan, 1991) were used.