Nature Communications (Mar 2021)
A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies
- Zhong Yao,
- Luka Drecun,
- Farzaneh Aboualizadeh,
- Sun Jin Kim,
- Zhijie Li,
- Heidi Wood,
- Emelissa J. Valcourt,
- Kathy Manguiat,
- Simon Plenderleith,
- Lily Yip,
- Xinliu Li,
- Zoe Zhong,
- Feng Yun Yue,
- Tatiana Closas,
- Jamie Snider,
- Jelena Tomic,
- Steven J. Drews,
- Michael A. Drebot,
- Allison McGeer,
- Mario Ostrowski,
- Samira Mubareka,
- James M. Rini,
- Shawn Owen,
- Igor Stagljar
Affiliations
- Zhong Yao
- Donnelly Centre, University of Toronto
- Luka Drecun
- Donnelly Centre, University of Toronto
- Farzaneh Aboualizadeh
- Donnelly Centre, University of Toronto
- Sun Jin Kim
- Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah
- Zhijie Li
- Department of Molecular Genetics, University of Toronto
- Heidi Wood
- Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada
- Emelissa J. Valcourt
- Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada
- Kathy Manguiat
- Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada
- Simon Plenderleith
- Biological Sciences, Sunnybrook Research Institute
- Lily Yip
- Biological Sciences, Sunnybrook Research Institute
- Xinliu Li
- Department of Microbiology, Mount Sinai Hospital
- Zoe Zhong
- Department of Microbiology, Mount Sinai Hospital
- Feng Yun Yue
- Department of Laboratory Medicine and Pathobiology, University of Toronto
- Tatiana Closas
- Canadian Blood Services
- Jamie Snider
- Donnelly Centre, University of Toronto
- Jelena Tomic
- Donnelly Centre, University of Toronto
- Steven J. Drews
- Canadian Blood Services
- Michael A. Drebot
- Zoonotic Diseases and Special Pathogens Division, National Microbiology Laboratory, Public Health Agency of Canada
- Allison McGeer
- Department of Microbiology, Mount Sinai Hospital
- Mario Ostrowski
- Department of Laboratory Medicine and Pathobiology, University of Toronto
- Samira Mubareka
- Biological Sciences, Sunnybrook Research Institute
- James M. Rini
- Department of Biochemistry, University of Toronto
- Shawn Owen
- Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah
- Igor Stagljar
- Donnelly Centre, University of Toronto
- DOI
- https://doi.org/10.1038/s41467-021-22102-6
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 8
Abstract
Serological tests are important diagnostic and disease surveillance tools for addressing the COVID-19 pandemic. Here, the authors present a tri-part Nanoluciferase based assay to detect antibodies against SARS-CoV-2.
