Investigating the effects of buforin II coding gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in kidney cancer cell line

Abstract

Introduction: Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults. The aim of this study is to investigate the effects of the buforin II gene on the expression of lncRNAs PVT1, EGOT and LINC00312 in ACHN kidney cancer cells. Material & Methods: Recombinant plasmid pcDNA3.1(+) containing buforin II gene and empty plasmid pcDNA3.1(+) were introduced into E. coli strain TOP10 by heat shock method and then purified. Then both mentioned plasmids were introduced into ACHN cells by lipofection method and screening was done by neomycin antibiotic. Finally, real time RT-PCR reaction was performed in order to investigate the expression change of EGOT, PVT1 and LINC00312 lncRNAs. Results: After lipofection, the transfected cells grew in the culture medium containing neomycin antibiotic. The real time RT-PCR reaction showed that the expression of buforin II gene in ACHN kidney cancer cells caused a significant increase in the expression of lncRNAs EGOT (P=0.0033) and LINC00312 (P=0.0272) and a significant decrease in the expression of PVT1 (P=0.0278). Discussion & Conclusion: Considering that the presence of buforin II gene causes a significant change in the expression of lncRNAs EGOT, PVT1 and LINC00312, it is possible that it can activate cell pathways including apoptosis.

Keywords

• renal cell carcinoma
• buforin ii
• achn cell line