Study on extraction technology and antioxidant activity of total alkaloids from Hemsleya chinensis based on orthogonal design and BP neural network
Weiwei Jiang,
Shaoyu Zheng,
Chengxiao Yuan,
Qingqing Gao,
Chunfan Xiang,
Shunwei Tian,
Jianmei Li,
Yan Zhao
Affiliations
Weiwei Jiang
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China; Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, 650106, China
Shaoyu Zheng
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
Chengxiao Yuan
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China; College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan, 650201, China
Qingqing Gao
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China; College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan, 650201, China
Chunfan Xiang
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China; College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan, 650201, China
Shunwei Tian
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
Jianmei Li
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China
Yan Zhao
National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China; Yunnan Characteristic Plant Extraction Laboratory, Kunming, Yunnan, 650106, China; College of Agronomy and Biotechnology, Yunnan Agricultural University, Yunnan, 650201, China; Corresponding author. National-Local Joint Engineering Research Center on Gemplasm Innovation & Utilization of Chinese Medicinal Materials in Southwest, The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming, Yunnan, 650201, China.
In this study, total alkaloids from Hemsleya chinensis were extracted and tested for their antioxidant properties. To optimize extraction methods, a single factor experiment was conducted to determine the total alkaloid concentrations of H. chinensis using the L9 (34) orthogonal design test method and the BP neural network (BPNN), resulting in the optimum extraction conditions for total alkaloids. The optimal conditions for H. chinensis alkaloids extraction with acid water are: HCl concentration is 0.50 %, extraction temperature is 85 °C, material-liquid ratio is 1:64.5, and extraction rate of alkaloids is 0.2785 ± 0.0003 mg/mL. The alkaloid from H. chinensis exhibited antioxidant activity in a quantity-effect relationship with activity. These findings showed that the procedure to be reasonable, the alkaloid extraction efficiency to be high, and the method could be used to extract the alkaloids of H. chinensis, improving the development of natural and healthy medicinal resources for the pharmaceutical and food industries.
